If you encounter this, simply compensate the data in FlowJo and the gates will automatically appear on the data once compensation is applied and the compensated version of the parameters exist.ДЈ) Nuances to gating and algorithmic approaches to automation

If gates, graphs or statistics reference the compensated version of collected parameters and uncompensated data are added to the template, none of the group associated gates will appear until the data are compensated. For that reason, we do not include compensation matrices as part of a template. Quality group creation is the key to automation.Дў) Compensation is independentВ of the templateД¬ompensation matrices will vary from experiment to experiment, even if the same panel has been used. If the group is live and has criteria, when new data are added to the template they will be automatically assigned to all groups with matching criteria, receive the gates associated with those groups, and connect to layouts and tables associated with those groups. Additional automation can be achieved by ensuring that the вЂLive groupвЂ™ check box is selected and that criteria have been attributed to define group membership.ДЄdditional information on creating groups is available on the Creating and Editing Groups page. Individual samples are not preserved in a template, so only gates that have been associated with a group will be retained. This is the most important part of designing the template. There are several tips that can improve the templates you create.

Templates can be used to apply the complete analysis to any subsequent data sets with a matching set of parameters. A template can be created simply by choosing the вЂExport as a templateвЂ™ option from the File band after analyzing an initial data set. It preserves groups, gates, table definitions, and layouts and allows all of that to be applied to the next data set. They are the most efficient tool for repeated analyses.ДЄ template is a replica of your workspace file without data. Templates contain your entire analysis, minus the FCS files.

Flowjo trial version use own samples